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SECTION II
PRIMERS FOR MAMMALIAN CLONING/SEQUENCING/EXPRESSION ANALYSES

The primers in this section have been designed for the viral vectors commonly used for cloning and expression of  therapeutic proteins in mammalian cell culture and also in gene therapy research.  Recommended regulatory guidelines for the characterization of cell lines used to express proteins frequently involves an examination of the potential heterogeneity of the mRNA transcripts in the master cell bank and the manufacturer’s working cell bank.  Amplification and sequencing data on mRNA from cells transformed or infected with the respective viral vectors can be obtained using the primer sets listed in this section.  Our primer pairs may be used to amplify and sequence mRNA during the production of a virus preparation used in gene therapy.  These analyses may be required for initial characterization of master and working cell/virus banks and for ongoing stability studies.  Characterization (amplification, sequencing) of the viruses and mRNA species present in tissues derived from animals used in efficacy and toxicity testing may also be performed using the relevant primer sets listed below.

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SV40 PRIMERS

SV40 Set 1 Early mRNA; 596bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
SV40early F eM-N021 GCC TCG GCC TCT GCA TAA ATA AAA (24) 2.5 nmol
SV40early R eM-N022 AAA TTG TGA CAA CCC CCT GGA TTA (24) 2.5 nmol


SV40 Set 2 3’ terminus; 573bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
SV403’end F eM-N023 GTA GTT TGT CCA ATT ATG TCA CAC (24) 2.5 nmol
SV403’end R eM-N024 AGT GAT GAA GAC CTT ATC GAG TCT (24) 2.5 nmol


SV40 Set 3 Late 16s intron; 725bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
SV40late F eM-N025 ACT GTT GGG GGA CCT AAT TGC TAC T (25) 2.5 nmol
SV40late R eM-N026 TAT AGT AAA CCC GTG TGG ATA CTA T (25) 2.5 nmol


ADENO-ASSOCIATED VIRUS TYPE 1 (AAV1) PRIMERS

AAV1 Set 1 647bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AAV1aF eM-N063 CAA AGG TCC GCA GAC GGC AGA G (22) 2.5 nmol
AAV1aR eM-N064 TGT TCG ACC ACG TCT GGT AGA T (22) 2.5 nmol


AAV1 Set 2 658bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AAV1bF eM-N065 AGG AGG ACC AGG CCT CGT ACA T (22) 2.5 nmol
AAV1bR eM-N066 TAC TGA AAC CGT TCC ACT GTT T (22) 2.5 nmol


AAV1 Set 3 657bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AAV1cF eM-N067 CAA CCT CGG GCG AGC AGT CTT C (22) 2.5 nmol
AAV1cR eM-N068 CAG TGC TGC TTA CTA CCG CAG TG (23) 2.5 nmol


AAV1 Set 4 637bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AAV1dF eM-N069 CAG TCC GGA AGT GCC CAA AAC AA (23) 2.5 nmol
AAV1dR eM-N070 CTT AGG AGG CCG CCT CAA AAG TC (23) 2.5 nmol

ADENO VIRUS TYPE 2 (AV2) PRIMERS

AV2 Set 1 577bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AV2 5’F eM-N071 CTT ATT TTG GAT TGA AGC CAA TAT (24) 2.5 nmol
AV2 5’R eM-N072 ATA GAC GGT GCC TCC ACA ATA ATG (24) 2.5 nmol


AV2 Set 2 526bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AV2 12sF eM-N073 TTC TCC TCC GAG CCG CTC CGA CAC (24) 2.5 nmol
AV2 12sR eM-N074 ACC AGT AAT AGT GGC CTC CTT ATG (24) 2.5 nmol



AV2 Set 3 752bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AV2 TAF eM-N075 GTC ACC AGG CGC TTT TCC AAG AGA (24) 2.5 nmol
AV2 TAR eM-N076 CTA TGC CCTC CTA TCC CAC CGG AAA T (25) 2.5 nmol


AV2 Set 4 605bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AV2 polF eM-N077 TCT ACG GCA TCT CGA TCC AGC ATA T (25) 2.5 nmol
AV2 polR eM-N078 CTC TCC GGA CAG GAG CTC GCC ACA A (25) 2.5 nmol


AV2 Set 5 742bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AV2 HEXF eM-N079 GAT GAT GCC GCA GTG GTC TTA CAT G (25) 2.5 nmol
AV2 HEXR eM-N080 GAT ACC TAG AAT ACG GTC CGG ATG T (25) 2.5 nmol


ADENO VIRUS TYPE 5 (AV5) PRIMERS

AV5 Set 1 623 bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AV5 P1F eM-N035 AAG AGG CCA CTC TTG AGT GCC AGC GAG TAG (30) 2.5 nmol
AV5 P1R eM-N036 ATT CAC TTT TAA TAC CCG TCA CCC ACT ATC T (31) 2.5 nmol


AV5 Set 2 1056 bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AV5 TF eM-N037 AAG AGG CCA CTC TTG AGT GCC AGC GAG TAG (30) 2.5 nmol
AV5 TR eM-N038 CAC ATT TGG ACA CTA ACG CAC ACA CCA ATT G (31) 2.5 nmol


AV5 Set 3 429 bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AV5 P2F eM-N039 TAC AGT TTT GTG GTT TAA AGA ATT TTG TAT T (31) 2.5 nmol
AV5 P2R eM-N040 CAC ATT TGG ACA CTA ACG CAC ACA CCA ATT G (31) 2.5 nmol


AV5 Set 4 3704 bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
AV5 E3F eM-N041 CAC ACC TCG TAA TAA CCT TAA TCC CCG TAG T (31) 2.5 nmol
AV5 E3R eM-N042 CTA TGG AAG TTG GGG CAC ATA GGT AGA CTG T (31) 2.5 nmol


VACCINIA VIRUS PRIMERS

Vaccinia virus Set 1 675 bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
VacciniaAF eM-N081 GAG ACC ATC GAA GGA GAA AGA GAT A (25) 2.5 nmol
VacciniaAR eM-N082 CTC TGG TAG CTT CCT CTT TCT CTA T (25) 2.5 nmol


Vaccinia virus Set 2 588 bp product

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
VacciniaBF eM-N083 GAC TCC ATC AGA AAG AGG TTT AAT A (25) 2.5 nmol
VacciniaBR eM-N084 CTG AGG TAG TCT TTC TCC AAA TTA T (25) 2.5 nmol


  • We will supply each oligonucleotide as desalted, lyophilized material.

  • Discounts apply to orders for multiple probe/primer sets.

  • We will ship by standard overnight or 2nd-day delivery (your choice).
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