Home  |  News  |  Products  |  Purchase  |  Contact  |  R&D About Us 
 
 
 
SECTION I
Primers for microbial cloning, sequencing and mutagenesis

The following universal primers are used to sequence or amplify inserts cloned into sequencing vectors or bacterial expression plasmids.

PRICE:  $12/tube  (call for quantity discounts)


M13/pUC PRIMERS

M13/pUC Set 1

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
M13/pUC F1 eM-N005 CAG TCA CGA CGT TGT AAA AC (20) 2.5 nmol
M13/pUC R1 eM-N006 TCA CAC AGG AAA CAG CTA TG (20) 2.5 nmol


M13/pUC Set 2

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
M13/pUC F2 eM-N007 GTT TTC CCA GTC ACG ACG TTG TAA A (25) 2.5 nmol
M13/pUC R2 eM-N008 AAT TGT GAG CGG ATA ACA ATT TCA C (25) 2.5 nmol

 

T3 PRIMERS

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
T3-ga eM-N009 AAT TAA CCC TCA CTA AAG (18) 2.5 nmol
T3-gb eM-N010 AAT TAA CCC TCA CTA AAG GGA (21)

2.5 nmol
T3-gc eM-N011 GCG CGA AAT TAA CCC TCA CTA AAG (24) 2.5 nmol



T7 PRIMER

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
T7-a eM-N012 TAA TAC GAC TCA CTA TAG GG (20) 2.5 nmol



SP6 PRIMERS

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
SP6-ga eM-N013 ATT TAG GTG ACA CTA TAG (18) 2.5 nmol
SP6-gb eM-N014 CAT ACG ATT TAG GTG ACA CTA TAG (24) 2.5 nmol



Ptac Sequencing Primer

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
Ptac-a  eM-N043 GAG CGG ATA ACA ATT TCA CAC AGG (24) 2.5 nmol



lgt 10 PRIMERS

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
lgt 10F eM-N044 CTT TTG AGC AAG TTC AGC CTG GTT AAG (27) 2.5 nmol
lgt 10R eM-N045 GAG GTG GCT TAT GAG TAT TTC TTC CAG GGT A (31) 2.5 nmol



lgt 11 PRIMERS

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
lgt 11F eM-N015 GGT GGC GAC GAC TCC TGG AGC CCG (24) 2 nmol
lgt 11R eM-N016 TTG ACA CCA GAC CAA CTG GTA ATG (24) 2 nmol



Repair and Knockout PRIMERS
PRICE:  $30/tube  (call for quantity discounts)

Set 1 – Ampicillin

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
Amp Rep eM-N027 GAC ATC GTT ACC GTT GTT GCA ACG CGT TTG (30) 5 nmol
Amp K/O eM-N028 GAC ATC GTT TTG CAA CGC GTT TG (23) 5 nmol



Set 2 - Tetracycline

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
Tet Rep eM-N029 CTC TTG CGG GAT ATC GTC CAT TCC GAC AGC (30) 5 nmol
Tet K/O eM-N030 CTC TTG CCG TCC ATT CCG ACA GC (23) 5 nmol



Set 3A – Chloramphenicol

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
Chlr Rep1 eM-N059 AAT TCC GGA TGA GCA TTC ATC AGG CGG GCA AGA (33) 5 nmol
Chlr K/O1 eM-N060 AAT TCC GGT TCA TCA GGC GGG CAA GA (26) 5 nmol



Set 3B - Chloramphenicol

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
Chlr Rep2 eM-N061 TAT CAC CAG CTC ACC GTC TTT CAT TGC CAT ACG  (33) 5 nmol
Chlr K/O2 eM-N062 TAT CAC CAG CTC TTC ATT GCC ATA CG (26)  5 nmol



Set 4A - Neomycin/Kanamycin

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
Neo Rep1 eM-N031 CAA AAA GAA CCG GGC GCC CCT GCG CTG ACA (30) 5 nmol
Neo K/O1 eM-N032 CAA AAA GAA CCC CTG CGC TGA CA (23)  5 nmol



Set 4B - Neomycin/Kanamycin

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
Neo Rep2 eM-N033 TCA GAA GAA CTC GTC AAG AAG GCG ATA GAA (30) 5 nmol
Neo K/O2 eM-N034 TCA GAA GAA AGA AGG CGA TAG AA (23) 5 nmol


RANDOM PRIMERS

Random primers are useful in the identification of variation among species through RAPD (Random Amplified Polymorphic DNA) analysis.  Random primers are used to amplify genomic regions for analysis of polymorphisms.  The protocol used involves denaturation at 95oC for 5 min followed by cycles at 95oC for 30 sec, 30oC for 2 min and 72oC for 1min.  This should be carried out for 33 cycles with the annealing temperature changed to 40oC.  The final cycle the extension step should be for 5 min (Hamid, et. al., 1999, Rev. Inst. Med. Trop. S. Paulo; Vol. 41, no. 5).  Longer random primers typically detect fewer polymorphisms and are best suited for more distantly related species.                                                                                                                                  PRICE:  $60/tube  (call for quantity discounts)

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
(dN)6  eM-N017 random mixture of all 4 nucleotides at each position (6) 1 OD260 unit
(dN)9 eM-N018 random mixture of all 4 nucleotides at each position (9) 1 OD260 unit
(dN)12 eM-N019 random mixture of all 4 nucleotides at each position (12) 1 OD260 unit
(dN)18 eM-N046 random mixture of all 4 nucleotides at each position (18) 1 OD260 unit
(dN)24 eM-N047 random mixture of all 4 nucleotides at each position (24) 1 OD260 unit
(dN)36 eM-N020 random mixture of all 4 nucleotides at each position (36) 1 OD260 unit


MOLECULAR WEIGHT/SIZE MARKERS

These oligonucleotides can be used as molecular weight/size markers to determine the size of PCR products or DNA inserts.

PRICE:  $10/tube  (call for quantity discounts)

NAME CAT. # SEQUENCE (LENGTH) AMOUNT
(dT)10 eM-N048 oligo-dT (10) 1 OD260 unit
(dT)20 eM-N049 oligo-dT (20) 1 OD260 unit
(dT)30 eM-N050 oligo-dT (30) 1 OD260 unit
(dT)40 eM-N051 oligo-dT (40) 1 OD260 unit
(dT)50 eM-N052 oligo-dT (50) 1 OD260 unit
(dT)60 eM-N053 oligo-dT (60) 1 OD260 unit
(dT)70 eM-N054 oligo-dT (70) 1 OD260 unit
(dT)80 eM-N055 oligo-dT (80) 1 OD260 unit
(dT)90 eM-N056 oligo-dT (90) 1 OD260 unit
(dT)100 eM-N057 oligo-dT (100) 1 OD260 unit
(dT)10 -  (dT)100 eM-N058 mixture of oligo-dT (10) through oligo-dT (100) 1 OD260 unit
 
  • We will supply each oligonucleotide as desalted, lyophilized material.

  • Discounts apply to orders for multiple probe/primer sets.

  • We ship via FedEx standard overnight or 2nd-day delivery (your choice).
 Online Catalog
Click here to place an order from our catalog.

Or send an email to: support@geno-mechanix.com with the products and quantities desired.


 






 
 

 
   
  Home  |  News  |  Products  |  Purchase  |  Contact  |  R&D About Us  
Back to Top

© 2006 GenoMechanix, LLC.